Journal: Frontiers in Microbiology

Article Title: In vitro diagnostic methods of Chagas disease in the clinical laboratory: a scoping review

doi: 10.3389/fmicb.2024.1393992

Figure Lengend Snippet: Characteristics of commercially available serological test included.

Article Snippet: In the United States, Chagatest ELISA recombinante v.3.0 from Wiener Lab, the Ortho® T. cruzi ELISA Test system from QuidelOrtho, and the Chagas Kit Elisa from Hemagen are the only approved ELISAs by the FDA.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Purification, Diagnostic Assay, Western Blot

Journal: Frontiers in Microbiology

Article Title: In vitro diagnostic methods of Chagas disease in the clinical laboratory: a scoping review

doi: 10.3389/fmicb.2024.1393992

Figure Lengend Snippet: Summary of process and performance of serological methods included in the analysis. Performance is reported as the mean sensitivity and specificity of included studies. Only the performance of commercial kits is reported, regardless of the antigen. Western blot only reports the performance of the Chagas Western Blot IgG assay. * Western Blot performance data derives from a single study.

Article Snippet: In the United States, Chagatest ELISA recombinante v.3.0 from Wiener Lab, the Ortho® T. cruzi ELISA Test system from QuidelOrtho, and the Chagas Kit Elisa from Hemagen are the only approved ELISAs by the FDA.

Techniques: Western Blot

Journal: Frontiers in Microbiology

Article Title: In vitro diagnostic methods of Chagas disease in the clinical laboratory: a scoping review

doi: 10.3389/fmicb.2024.1393992

Figure Lengend Snippet: Summary of process and performance of molecular methods included in the analysis. All test types were included regardless of their commercial status due to the little number of commercially available kits. Performance is reported as the mean sensitivity and specificity of included studies. a cPCR: Most studies included used cPCR for chronic Chagas diagnosis, and one included study used cPCR in acute Chagas samples, which explains this sensitivity value . b ddPCR: performance data derives from a single study. c LAMP is discontinued.

Article Snippet: In the United States, Chagatest ELISA recombinante v.3.0 from Wiener Lab, the Ortho® T. cruzi ELISA Test system from QuidelOrtho, and the Chagas Kit Elisa from Hemagen are the only approved ELISAs by the FDA.

Techniques:

Journal: PLOS Neglected Tropical Diseases

Article Title: Diversity of Chagas disease diagnostic antigens: Successes and limitations

doi: 10.1371/journal.pntd.0012512

Figure Lengend Snippet: Application of defined antigens in commonly reported commercialised tests.

Article Snippet: 2019 , Whitman et al. [ ] , Blood donors originating from MEX, Central America, or South America , RDT: CDP. ELISA: Ortho T . cruzi ; Hemagen Chagas kit ; Chagatest Recombinante v.3.0. , (i) Immunoassays or RIPA used for American Red Cross blood donations (BD); (ii) consensus from this study; (iii) latent class analysis (modelling). , Compared to BD or consensus status, sensitivity for Ortho T . cruzi , Chagatest Recombinante, and Hemagen tended to lowest in MEX-born, intermediate in Central Americans and highest in South Americans; trend consistent for seropositives by the 4 assays..

Techniques: Enzyme-linked Immunosorbent Assay

Journal: PLOS Neglected Tropical Diseases

Article Title: Diversity of Chagas disease diagnostic antigens: Successes and limitations

doi: 10.1371/journal.pntd.0012512

Figure Lengend Snippet: Reports of varying geographical sensitivity of Chagas disease serological tests (2008–2021).

Article Snippet: 2019 , Whitman et al. [ ] , Blood donors originating from MEX, Central America, or South America , RDT: CDP. ELISA: Ortho T . cruzi ; Hemagen Chagas kit ; Chagatest Recombinante v.3.0. , (i) Immunoassays or RIPA used for American Red Cross blood donations (BD); (ii) consensus from this study; (iii) latent class analysis (modelling). , Compared to BD or consensus status, sensitivity for Ortho T . cruzi , Chagatest Recombinante, and Hemagen tended to lowest in MEX-born, intermediate in Central Americans and highest in South Americans; trend consistent for seropositives by the 4 assays..

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Recombinant, Enzyme-linked Immunospot